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Larval salivary gland chromosomes of the blackfly, Simulium (Gomphostilbia) sundaicum (Diptera : Simuliidae) from Java, Indonesia

Posted by on November 3, 2011

Larval salivary gland chromosomes of the blackfly, Simulium (Gomphostilbia) sundaicum (Diptera : Simuliidae) from Java, Indonesia.

Medical Entomology and Zoology 47(1), 55-61, 1996, The Japan Society of Medical Entomology and Zoology.

Hadi,Upik Kesumawati, Hiroyuki Takaoka, Kashiko Kondo & Hirohisa Hirai

Abstract: Polytene chromosome study of the Oriental Simuliidae has been only scantily made. Larval salivary gland chromosomes of the blackfly, Simulium (Gomphostilbia) sundaicum Edwards, 1934,from Java, Indonesia, were observed and their standard maps were constructed. This species had three pairs of polytene chromosomes. The chromosome I and II were metacentric and the chromosome III was submetacentric. Each centromere consisted of a large conspicuous heterochromatic segment in all chromosomes. The large heterochromatin was associated with each other in some cells. The nucleolar organizer was located in section 19 of chromosome I. The Balbiani ring, the double bubble and the Parabalbiani ring were situated in section 49C, 48 and 64C of chromosome II, respectively. Five inversions were found, but none of them was sex-related. C-band staining successfully revealed that band of 84A, centromeric of chromosome III, was heterozygously stained, slightly and heavily in males, while homozygously stained, only heavily in females. Then the slightly stained band could be ascribed to Y-chromosomal, and the heavily stained, to X-chromosomal.

 

Full Paper in PDF: Larval salivary gland chromosomes of the blackfly, Simulium (Gomphostilbia) sundaicum (Diptera Simuliidae) from Java, Indonesia.

 

Title in Japanese: インドネシア国ジャワ島産ブユ, Simulium (Gomphostilbia) sundaicum 幼虫の唾液腺染色体の観察.

東洋区産ブユについての染色体の研究はほとんどない。今 回, インドネシア国ジャワ島産ブユSimulium (Gomphostilbia) sundaicum幼虫の唾液腺染色体を観察し, 基本となる染色体マップを作製した。本種の唾液腺染色体は相同染色体が対合した3本(2n=6)からなり, ヘテロクロマチンに富む明瞭な動原体部を有していた。主な特徴のうち, 仁形成部は第一染色体に, また, バルビアニ環および副バルビアニ環は第二染色体に位置していた。逆位は5種観察されたが, いずれも性に連鎖していなかった。性連鎖に関係したバンディングを見るため, C-banding法を試みたところ, 第三染色体の動原体に見られる84Aバンドが, 雄では濃淡ヘテロ, 雌では濃色ホモで見られたため, 濃淡バンドはそれぞれ, X, Y染色体性をもつと考えられた

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