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Bioekologi vektor demam berdarah dengue (DBD) serta deteksi virus dengue pada Aedes aegypti (Linnaeus) dan Ae. albopictus (Skuse) (Diptera: Culicidae) di kelurahan endemik DBD Bantarjati, Kota Bogor

Posted by on November 9, 2015

Zahara Fadilla, Upik Kesumawati Hadi*, Surachmi Setiyaningsih Program Studi Parasitologi dan Entomologi Kesehatan, Institut Pertanian Bogor Jalan Agatis Kampus IPB Darmaga, Bogor 16680.

Jurnal Entomologi Indonesia, Vol. 12 No. 1, 31–38 DOI: 10.5994/jei.12.1.31

Bioekologi vektor demam berdarah dengue (DBD) serta deteksi virus dengue pada Aedes aegypti (Linnaeus) dan Ae. albopictus (Skuse) (Diptera: Culicidae) di kelurahan endemik DBD Bantarjati, Kota Bogor. [Bioecology of dengue haemorrhagic fever vectors and dengue virus detection in Aedes aegypti (Linnaeus) and Ae. albopictus (Skuse) (Diptera: Culicidae) in endemic area of Bogor City]

ABSTRAK: Demam berdarah dengue (DBD) merupakan salah satu penyakit yang disebabkan oleh virus dengue dan masih menjadi masalah kesehatan masyarakat di Indonesia. Penelitian ini dilakukan untuk mengetahui bioekologi Aedes spp. dan deteksi virus dengue pada nyamuk Aedes spp. di Kelurahan Bantarjati Kota Bogor. Penangkapan nyamuk dilakukan bulan April sampai Juli 2012. Pada nyamuk Aedes spp. dilakukan deteksi virus Dengue dengan teknik reverse transcriptionpolymerase chain reaction (RT-PCR) yang terdiri dari 2 tahap, yaitu tahap sintesis dan amplifikasi CDNA serta tahap karakterisasi serotipe virus dengue. Penangkapan nyamuk Aedes aegypti (Linnaeus) dan Ae. albopictus (Skuse) dilakukan pada 200 rumah penduduk di daerah endemik DBD dengan metode umpan orang di dalam dan luar rumah. Kepadatan nyamuk Ae. aegypti dan Ae. albopictus tertinggi terjadi pada bulan April dengan puncak aktivitas mengisap darah pada jam 10:00-11:00. Virus dengue tidak terdeteksi pada nyamuk betina Aedes spp. yang ditangkap di Kelurahan Bantarjati Kota Bogor.

Kata kunci: Aedes spp., demam berdarah dengue, virus dengue, polymerase chain reaction.

 
ABSTRACT: Dengue hemorrhagic fever (DHF) is a viral disease that threatened community health in Indonesia. As part of an eradication program, it is important to learn the behavioral aspect of the disease vector. The aims of this study were to detect the presence of dengue virus in Aedes spp, at Bantarjati Village, Bogor City and to learn to bioecology of. Aedes aegypti (Linnaeus). Detection of dengue virus in Aedes spp. were done by reverse transcription-polymerase chain reaction (RT-PCR) technique that consist of two phase were synthesis phase and cDNA amplification and dengue virus serotipe characterization. The Ae. aegypti and Ae. albopictus (Skuse) mosquitoes were collected using the landing and resting moquito collection technique booth indoors and outdoors. The highest density of Ae. aegypti and Ae. albopictus were found in April and the peak activity was occurred at 10:00-11:00 am. Dengue virus was not detected in female mosquitoes Aedes spp.

Key word: Aedes spp., dengue hemorrhagic fever, polymerase chain reaction

Full Paper in PDF: Bioecology of dengue haemorrhagic fever vectors and dengue virus detection in Aedes aegypti and Ae. albopictus in endemic area of Bogor City

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