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Cytogenetic and Molecular Evidence of Additional Cryptic Diversity in High Elevation Black fly Simulium feuerborni (Diptera: Simuliidae) Populations in Southeast Asia

Posted by on November 27, 2015

Cytogenetic and Molecular Evidence of Additional Cryptic Diversity in High Elevation Black fly Simulium feuerborni (Diptera: Simuliidae) Populations in Southeast Asia

Journal of Medical Entomology 52(5):829-836. 2015
doi: http://dx.doi.org/10.1093/jme/tjv080

  • Pairot Pramual,1,2,3Jiraporn Thaijarern,1Mohd Sofian-Azirun,4 Zubaidah Ya’Cob,4 Upik Kesumawati Hadi,5 and Hiroyuki Takaoka4

1 Department of Biology, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham 44150, Thailand.

2 Biodiversity and Traditional Knowledge Research Unit, Faculty of Science, Mahasarakham University, Kantharawichai District, Maha Sarakham 44150, Thailand.

4 Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, 50603, Malaysia.

5 Division of Parasitology and Medical Entomology, Faculty of Veterinary Medicine, Bogor Agricultural University, Bogor, Indonesia. Jl Agatis Kampus IPB Darmaga Bogor 16680.

3 Corresponding author, e-mail: pairot.p@msu.ac.th.

 

ABSTRACT

Simulium feuerborni Edwards is geographically widespread in Southeast Asia. Previous cytogenetic study in Thailand revealed that this species is a species complex composed of two cytoforms (A and B). In this study, we cytologically examined specimens obtained from the Cameron Highlands, Malaysia, and Puncak, Java, Indonesia. The results revealed two additional cytoforms (C and D) of S. feuerborni. Specimens from Malaysia represent cytoform C, differentiated from other cytoforms by a fixed chromosome inversion on the long arm of chromosome III (IIIL-5). High frequencies of the B chromosome (33–83%) were also observed in this cytoform. Specimens from Indonesia represent the cytoform D. This cytoform is differentiated from others by a fixed chromosome inversion difference on the long arm of chromosome II (IIL-4). Mitochondrial DNA sequences support genetic differentiation among cytoforms A, B, and C. The pairwise FST values among these cytoforms were highly significantly consistent with the divergent lineages of the cytoforms in a median-joining haplotype network. However, a lack of the sympatric populations prevented us from testing the species status of the cytoforms.

Keywords: polytene chromosomeSimuliidaeSimulium species complex

ABSTRACT IN PDFCytogenetic and Molecular Evidence of Additional Cryptic Diversity in High Elevation Black fly Simulium feuerborni (Diptera Simuliidae) Populations in Southeast Asia (Abstract)

 

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Journal of Medical Entomology

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